Laser 2000
Home > Products > Light Delivery & Control > Microscopy Filters > Filter Sets & Cubes > Single-Band Microscopy Sets > BrightLine single-band filter set: Cy3 and similar fluorophores

BrightLine single-band filter set: Cy3 and similar fluorophores

Semrock Logo

This filter set is ideal for use with Cy3™ and a variety of other orange fluorophores (including DsRed fluorescent protein). It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio.

Are you co-labeling with YFP, GFP, or FITC? In order to substantially eliminate green-fluorophore bleedthrough we recommend using our TRITC-B filter set. For mercury (e.g., HBO) lamp users labeling with Cy3 the single-fluorophore signal-to-noise ratio will be slightly higher, and the brightness slightly lower, with the TRITC-A filter set. Xenon lamp users of the TRITC-A filter set who are labeling with Cy3 will enjoy a significantly enhanced signal-to-noise ratio and reduced photobleaching in exchange for brightness.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts - please contact us for custom sizes)
Filter sizeOlympus 'Large' (FX)
SizeOlympus large-size (FX) set for U-M710I
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.

Alexa Fluor 555
Calcium Orange
LysoTracker Yellow
MitoTracker Orange
Rhodamine Phalloidin


  • Size: Olympus large-size (FX) set for U-M710I

Cy3-4040C-U02 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-531/40-32 Single Band Exciter 32 x 5.0 mm 1
FF01-593/40-32 Single Band Emitter 32 x 3.5 mm 1
FF562-Di03-32×44-FX Single Band Dichroic 32 x 44 x 1.1 mm 1


Loading chart data...

Optical Density

Loading chart data...

Semrock Spectral Plot Info

Request a Demo


Ask the Expert

If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.