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BrightLine long-pass filter set: most Qdot Nanocrystals and similar fluorophores

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A special long-pass emission filter in this filter set allows simultaneous viewing by eye or with a color camera of samples that are multi-labeled with quantum dot nanocrystals. It takes advantage of the unique fact that all quantum dots share a common UV/blue excitation spectrum while simultaneously exhibiting narrow and specific visible emission spectra. The highly transmitting, deep-blue exciter efficiently excites all quantum dots with no UV light to damage samples or generate undesirable background, while the high and uniform transmission of the long-pass emission filter provides exceptional and balanced brightness. And with the ‘no burn-out’ performance shared by all BrightLine filters, the permanent performance of this set will outlast even your quantum dots!

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
Filter sizeOlympus 'Large' (FX)
SizeOlympus large-size (FX) set for U-M710I
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreQdot 525
Qdot 565
Qdot 585
Qdot 605
Qdot 625
Qdot 655
Qdot 705
Qdot 800 Nanocrystals


QDLP-C-U02

  • Size: Olympus large-size (FX) set for U-M710I

QDLP-C-U02 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-435/40-32 Single Band Exciter 32 x 5.0 mm 1
FF01-500/LP-32 Long Pass Emitter 32 x 3.5 mm 1
FF510-Di02-32×44-FX Single Band Dichroic 32 x 44 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-500_LP_spectrum
FF02-435_40_Spectrum
FF510-Di02_Spectrum
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QDLP-C

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