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BrightLine® long-pass λ/2 flatness super-resolution laser filter set: 635 nm laser sources

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This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today’s state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from Cy5 and other similar fluorophores while offering superb blocking of popular 635 nm (solid-state laser diode), 633 nm (HeNe gas) or 647 nm (Kr-ion gas) lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= λ/2 PV per inch @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?

Our previous version is still available for those cube configurations LF635/LP-B

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts - please contact us for custom sizes)
Filter sizeStandard
SizeStandard size (fits most microscopes)
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.

FluorophoreAlexa Fluor 633
Alexa Fluor 647
BODIPY 650/665-X
DiD (DiIC18(5)) - Lipophilic Tracer


  • Size: Standard size (fits most microscopes)

LF635/LP-C-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-640/14-25 Single Band Exciter 25 x 5.0 mm 1
BLP01-635R-25 Single Band Emitter 25 x 3.5 mm 1
Di03-R635-t1-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1


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Optical Density

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Semrock Spectral Plot Info

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Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.