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BrightLine FRET filter set: Blue & Green Fluorescent Protein and similar fluorophores

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This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for blue donor fluorescence and green acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Sutter threaded set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreAlexa Fluor 350
Alexa Fluor 488
BFP
Cy2
DAPI
EGFP
FITC
GFP


FRET-BFP/GFP-B-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

FRET-BFP/GFP-B-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-387/11-25 Single Band Exciter 25 x 5.0 mm 1
FF02-447/60-25 Single Band Emitter 25 x 3.5 mm 1
FF01-520/35-25 Single Band Emitter 25 x 3.5 mm 1
FF409-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

FRET-BFP/GFP-B-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

FRET-BFP/GFP-B-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-387/11-25 Single Band Exciter 25 x 5.0 mm 1
FF02-447/60-25 Single Band Emitter 25 x 3.5 mm 1
FF01-520/35-25 Single Band Emitter 25 x 3.5 mm 1
FF409-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

FRET-BFP/GFP-B-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

FRET-BFP/GFP-B-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-387/11-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF02-447/60-25 Single Band Emitter 25 x 3.5 mm 1
FF01-520/35-25 Single Band Emitter 25 x 3.5 mm 1
FF409-Di03-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1

FRET-BFP/GFP-B-S01

  • Size: Sutter threaded set
  • Filter size: Sutter Threaded
  • Zero pixel shift: no

FRET-BFP/GFP-B-S01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-387/11-25 Single Band Exciter 25 x 5.0 mm 1
FF02-447/60-25-STR Single Band Emitter 25 mm 1
FF01-520/35-25-STR Single Band Emitter 25 mm 1
FF409-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

FRET-BFP/GFP-B-S01-ZERO

  • Filter size: Sutter Threaded
  • Zero pixel shift: yes

FRET-BFP/GFP-B-S01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-387/11-25 Single Band Exciter 25 x 5.0 mm 1
FF02-447/60-25-STR Single Band Emitter 25 mm 1
FF01-520/35-25-STR Single Band Emitter 25 mm 1
FF409-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-387_11_Spectrum
FF01-520_35_spectrum
FF02-447_60_spectrum
FF409-Di03_Spectrum
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FRET-BFP/GFP-B

Ask the Expert

If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.