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BrightLine Sedat laser filter set: 405, 488, & 594 nm laser sources

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This ‘Sedat’ multiband set is optimized for laser excitation utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, and a 593.5, 594.0 or 594.1 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreDAPI
FITC
GFP
mCherry
Texas Red


LF405/488/594-3X3M-B-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

LF405/488/594-3X3M-B-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-390/40-25 Single Band Exciter 25 x 5.0 mm 1
FF02-482/18-25 Single Band Exciter 25 x 5.0 mm 1
FF01-591/6-25 Single Band Exciter 25 x 5.0 mm 1
FF01-445/20-25 Single Band Emitter 25 x 5.0 mm 1
FF01-525/45-25 Single Band Emitter 25 x 3.5 mm 1
FF01-647/57-25 Single Band Emitter 25 x 3.5 mm 1
Di01-R405/488/594-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LF405/488/594-3X3M-B-L01

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’

LF405/488/594-3X3M-B-L01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-390/40-25 Single Band Exciter 25 x 5.0 mm 1
FF02-482/18-25 Single Band Exciter 25 x 5.0 mm 1
FF01-591/6-25 Single Band Exciter 25 x 5.0 mm 1
FF01-445/20-25 Single Band Emitter 25 x 5.0 mm 1
FF01-525/45-25 Single Band Emitter 25 x 3.5 mm 1
FF01-647/57-25 Single Band Emitter 25 x 3.5 mm 1
Di01-R405/488/594-22×29 Triple Band Dichroic 22.0 x 29.0 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-390_40_Spectrum
FF01-445_20_Spectrum
FF01-525_45_Spectrum
FF01-647_57_Spectrum
FF02-482_18_Spectrum
Di01-R405_488_594_Spectrum
FF01-591_6_Spectrum
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