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BrightLine Sedat filter set: FITC & Texas Red and similar fluorophores

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This 5-filter ‘Sedat’ set is ideal for high-speed sequential imaging FITC and Texas Red with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. It is also very popular for imaging GFP and mCherry. It is comprised of two single-band exciters and two single-band emitters, along with a dual-band beamsplitter, and thus works best when used with both exciter and emitter filter wheels.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Sutter threaded set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreAlexa Fluor 488
Alexa Fluor 568
Alexa Fluor 594
BODIPY
FITC
GFP
mCherry
mRFP1
rsGFP
Texas Red


FITC/TXRED-2X2M-B-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

FITC/TXRED-2X2M-B-000 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF02-485/20-25Single Band Exciter25 x 5.0 mm1
FF01-586/20-25x5Single Band Exciter25 x 5.0 mm1
FF01-536/40-25Single Band Emitter25 x 3.5 mm1
FF01-628/32-25Single Band Emitter25 x 3.5 mm1
FF505/606-Di01-25x36Dual Band Dichroic25.2 x 35.6 x 1.1 mm1

FITC/TXRED-2X2M-B-L01

  • Size: Leica 'large' set
  • Filter size: Leica 'Large'

FITC/TXRED-2X2M-B-L01 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF02-485/20-25Single Band Exciter25 x 5.0 mm1
FF01-586/20-25x5Single Band Exciter25 x 5.0 mm1
FF01-536/40-25Single Band Emitter25 x 3.5 mm1
FF01-628/32-25Single Band Emitter25 x 3.5 mm1
FF505/606-Di01-22x29Dual Band Dichroic22.0 x 29.0 x 1.1 mm1

FITC/TXRED-2X2M-B-S01

  • Size: Sutter threaded set
  • Filter size: Sutter Threaded

FITC/TXRED-2X2M-B-S01 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF02-485/20-25-STRSingle Band Exciter25 mm1
FF01-586/20-25-STRSingle Band Exciter25 mm1
FF01-536/40-25-STRSingle Band Emitter25 mm1
FF01-628/32-25-STRSingle Band Emitter25 mm1
FF505/606-Di01-25x36Dual Band Dichroic25.2 x 35.6 x 1.1 mm1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF505_606-Di01_Spectrum
FF01-536_40_spectrum
FF01-628_32_spectrum
FF01-586_20_spectrum
FF02-485_20_spectrum
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FITC/TXRED-2X2M-B

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If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.