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BrightLine® Sedat filter set: DAPI, FITC, & Texas Red and similar fluorophores

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This 7-filter ‘Sedat’ set is based on the award-winning BrightLine blue-green-red multiband filter set, but instead of multiband exciter and emitter filters, it has three single-band exciters, three single-band emitters, and a triple-band beamsplitter. With its high brightness, extremely low crosstalk, and superb signal-to-noise performance, it is ideal for three-fluorophore high-speed sequential imaging using a low-noise monochrome camera and filter wheels for both the exciters and the emitters.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Sutter threaded set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreAlexa Fluor 488
AMCA
BODIPY
DAPI
FITC
GFP
Hoechst 33258
Hoechst 33342
Hoechst 34580
MitoTracker Red
rsGFP
Texas Red


DA/FI/TX-3X3M-C-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

DA/FI/TX-3X3M-C-000 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-387/11-25Single Band Exciter25 x 5.0 mm1
FF01-494/20-25Single Band Exciter25 x 5.0 mm1
FF03-575/25-25Single Band Exciter25 x 5.0 mm1
FF02-447/60-25Single Band Emitter25 x 3.5 mm1
FF02-531/22-25Single Band Emitter25 x 5.0 mm1
FF01-624/40-25Single Band Emitter25 x 3.5 mm1
FF436/514/604-Di01-25x36Triple Band Dichroic25.2 x 35.6 x 1.1 mm1

DA/FI/TX-3X3M-C-L01

  • Size: Leica 'large' set
  • Filter size: Leica 'Large'

DA/FI/TX-3X3M-C-L01 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-387/11-25Single Band Exciter25 x 5.0 mm1
FF01-494/20-25Single Band Exciter25 x 5.0 mm1
FF03-575/25-25Single Band Exciter25 x 5.0 mm1
FF02-447/60-25Single Band Emitter25 x 3.5 mm1
FF02-531/22-25Single Band Emitter25 x 5.0 mm1
FF01-624/40-25Single Band Emitter25 x 3.5 mm1
FF436/514/604-Di01-22x29Triple Band Dichroic22.0 x 29.0 x 1.1 mm1

DA/FI/TX-3X3M-C-S01

  • Size: Sutter threaded set
  • Filter size: Sutter Threaded

DA/FI/TX-3X3M-C-S01 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-387/11-25-STRSingle Band Exciter25 mm1
FF01-494/20-25-STRSingle Band Exciter25 mm1
FF03-575/25-25-STRSingle Band Exciter25 mm1
FF02-447/60-25-STRSingle Band Emitter25 mm1
FF02-531/22-25-STRSingle Band Emitter25 mm1
FF01-624/40-25-STRSingle Band Emitter25 mm1
FF436/514/604-Di01-25x36Triple Band Dichroic25.2 x 35.6 x 1.1 mm1

Transmission

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Optical Density

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