Laser 2000
Home > Products > Light Delivery & Control > Microscopy Filters > Filter Sets & Cubes > Sedat Multiband Sets > BrightLine® Sedat λ/2 flatness super-resolution laser filter set: 405, 488, 561, & 635 nm laser sources

BrightLine® Sedat λ/2 flatness super-resolution laser filter set: 405, 488, 561, & 635 nm laser sources

Semrock Logo

This ‘Sedat’ multiband set is optimized for laser excitation over four bands – utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, a 559, 561.4 or 568.2 nm laser source for mCherry, and a 632.8, 635, or 647.1 nm laser source for Cy5. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?

Our previous version is still available for those cube configurations LF405/488/561/635-4X4M-A

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
Filter sizeStandard
SizeStandard size (fits most microscopes)
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreCy5
DAPI
FITC
GFP
mCherry
mRFP1


LF405/488/561/635-4X4M-B-000

  • Size: Standard size (fits most microscopes)

LF405/488/561/635-4X4M-B-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-390/40-25 Single Band Exciter 25 x 5.0 mm 1
FF02-482/18-25 Single Band Exciter 25 x 5.0 mm 1
FF01-563/9-25 Single Band Exciter 25 x 5.0 mm 1
FF01-640/14-25 Single Band Exciter 25 x 5.0 mm 1
FF01-445/20-25 Single Band Emitter 25 x 5.0 mm 1
FF01-525/30-25 Single Band Emitter 25 x 3.5 mm 1
FF01-605/15-25 Single Band Emitter 25 x 3.5 mm 1
FF01-676/29-25 Single Band Emitter 25 x 3.5 mm 1
Di03-R405/488/561/635-t1-25×36 Quad Band Dichroic 25.2 x 35.6 x 1.1 mm 1

Transmission

Loading chart data...

Optical Density

Loading chart data...