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BrightLine Pinkel filter set: Green Fluorescent Protein & DsRed and similar fluorophores

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This 4-filter ‘Pinkel’ set is perfectly optimized for imaging GFP and DsRed with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The set is also ideal for samples dual-labeled with FITC and TRITC, or GFP and Cy3, among other fluorophore combinations. It is comprised of two single-band exciters along with a dual-band beamsplitter and a dual-band emitter, and thus works best with an exciter filter wheel or slider.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Sutter threaded set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreAlexa Fluor 488
Alexa Fluor 568
Alexa Fluor 594
Cy3
DsRed
FITC
GFP
rsGFP
Texas Red-X Conjugate
TRITC


GFP/DsRed-2X-A-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

GFP/DsRed-2X-A-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-470/22-25 Single Band Exciter 25 x 5.0 mm 1
FF01-556/20-25 Single Band Exciter 25 x 5.0 mm 1
FF01-512/630-25 Dual Band Emitter 25 x 3.5 mm 1
FF493/574-Di01-25×36 Dual Band Dichroic 25.2 x 35.6 x 1.1 mm 1

GFP/DsRed-2X-A-L01

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’

GFP/DsRed-2X-A-L01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-470/22-25 Single Band Exciter 25 x 5.0 mm 1
FF01-556/20-25 Single Band Exciter 25 x 5.0 mm 1
FF01-512/630-23.3-D Dual Band Emitter 23.3 x 2.0 mm 1
FF493/574-Di01-22×29 Dual Band Dichroic 22.0 x 29.0 x 1.1 mm 1

GFP/DsRed-2X-A-S01

  • Size: Sutter threaded set
  • Filter size: Sutter Threaded

GFP/DsRed-2X-A-S01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-470/22-25-STR Single Band Exciter 25 mm 1
FF01-556/20-25-STR Single Band Exciter 25 mm 1
FF01-512/630-25 Dual Band Emitter 25 x 3.5 mm 1
FF493/574-Di01-25×36 Dual Band Dichroic 25.2 x 35.6 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-512_630_Spectrum
FF493_574-Di01_spectrum
FF01-470_22_spectrum
FF01-556_20_spectrum

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GFP/DsRed-2X-A

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Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.