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BrightLine®; full-multiband filter set: Cyan & Yellow Fluorescent Proteins and similar fluorophores, illuminated with Lumencor CELESTA light engine

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This ‘full-multiband’ filter set allows for simultaneous direct viewing or imaging of CFP (Cyan Fluorescent Protein) and YFP (Yellow Fluorescent Protein) (and other key fluorophores – see complete list above). This set has been optimized to be illuminated with Lumencor CELESTA light engine® utilizing 445 nm & 518 nm wavelengths. This set has been designed to deliver significant fluorescence signal improvements for the CELESTA light engine compared with sets designed for standard broadband light sources such as mercury or xenon arc lamps or other LED-based light engines. The complete set is comprised of a dual-band beamsplitter, emitter, and exciter filter, all with ‘no burn-out’ performance.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreCerulean
CFP
Venus
YFP


CELESTA-CFP/YFP-A-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

CELESTA-CFP/YFP-A-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-484/561-25 Dual Band Emitter 25 x 3.5 mm 1
FF01-449/520-25 Dual Band Exciter 25 x 5.0 mm 1
FF471/539-Di01-25×36 Dual Band Dichroic 25.2 x 35.6 x 1.1 mm 1

CELESTA-CFP/YFP-A-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

CELESTA-CFP/YFP-A-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-484/561-25 Dual Band Emitter 25 x 3.5 mm 1
FF01-449/520-25 Dual Band Exciter 25 x 5.0 mm 1
FF471/539-Di01-25×36 Dual Band Dichroic 25.2 x 35.6 x 1.1 mm 1

CELESTA-CFP/YFP-A-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

CELESTA-CFP/YFP-A-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-484/561-23.3-D Dual Band Emitter 23.3 x 2.0 mm 1
FF01-449/520-21.8-D Dual Band Exciter 21.8 x 2.0 mm 1
FF471/539-Di01-22×29 Dual Band Dichroic 22.0 x 29.0 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF471_539-Di01_Spectrum
FF01-449_520_Spectrum
FF01-484_561_Spectrum

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CELESTA-CFP/YFP-A

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