Laser 2000
Home > Products > Light Delivery & Control > Microscopy Filters > Filter Sets & Cubes > Full Multiband Sets > BrightLine® full-multiband filter set: CFP, YFP, & mCherry and similar fluorophores, illuminated with LED-based light engines

BrightLine® full-multiband filter set: CFP, YFP, & mCherry and similar fluorophores, illuminated with LED-based light engines

Semrock Logo

This Full-multiband triple-band set is designed for imaging CFP, YFP, and mCherry (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in triple-band performance, and has been optimized for popular LED-based light engines. This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter, emitter, and exciter filter, all with ‘no burn-out’ performance.

Interested in learning how this filter set will perform with your light source?

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreCerulean
CFP
mCherry
Texas Red
Venus
YFP


LED-CFP/YFP/mCherry-A-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

LED-CFP/YFP/mCherry-A-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-438/509/578-25 Triple Band Exciter 25 x 5.0 mm 1
FF01-475/543/702-25 Triple Band Emitter 25 x 3.5 mm 1
FF459/526/596-Di01-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-CFP/YFP/mCherry-A-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

LED-CFP/YFP/mCherry-A-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-438/509/578-25 Triple Band Exciter 25 x 5.0 mm 1
FF01-475/543/702-25 Triple Band Emitter 25 x 3.5 mm 1
FF459/526/596-Di01-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-CFP/YFP/mCherry-A-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

LED-CFP/YFP/mCherry-A-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-438/509/578-21.8-D Triple Band Exciter 21.8 x 2.0 mm 1
FF01-475/543/702-23.3-D Triple Band Emitter 23.3 x 2.0 mm 1
FF459/526/596-Di01-22×29 Triple Band Dichroic 22.0 x 29.0 x 1.1 mm 1

Transmission

Loading chart data...

Optical Density

Loading chart data...

Semrock Spectral Plot Info
FF01-475_543_702_Spectrum
FF459_526_596_Di01_Spectrum
FF01-438_509_578_Spectrum
BACK
TO TOP

Request a Demo

LED-CFP/YFP/mCherry-A

Ask the Expert

If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.