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niji LED Light Source for Fluorescence Microscopy – fully populated with 7 modules

Select HPUV model for high power UV option (330 mW at 390 nm).

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The niji is a revolutionary and innovative LED light source that is ideally suited to the demands of fluorescence microscopy.

With light emission from up to seven individually addressable, individually filtered LED channels that simultaneously emit light through a single combined light guide, the niji delivers powerful, multi-wavelength excitation from the UV to the NIR, specifically for single and multiband fluorescence microscopy.

Based around a uniquely reconfigurable mainframe, LEDs and filters can be changed by the user in minutes, offering additional excitation centre wavelengths, excitation bands, and output power options, dramatically expanding the capability of the niji.

Wavelength, power, and emission channel can all be controlled, individually or simultaneously, using the manual controller (tablet) or via software.

Constant current and constant optical power LED drive modes are both selectable, enabling fast imaging speed and high excitation power stability over long periods.

Software control options are numerous and the niji can be integrated into a microscope application in a variety of ways.

Fully compatible with MicroManager and MicroManager 2, the niji can also be operated with a wide range of industry standard microscope software packages (when operating in compatibility mode via RS232).

The niji is also mechanically compatible with most standard microscopes. Numerous adaptors, light guides, and collimators are available off the shelf, to help to simplify the task.

Furthermore, great care has been taken to make the niji one of the quietest LED sources on the market. With optimised fans running only when needed, you’ll be able to hear yourself think and work, knowing that vibrations at the microscope are minimised.

If funds are tight or you don’t know what wavelengths you might need in future, the niji provides the perfect solution, combining a low cost entry point with a low cost upgrade path.

To find out more about the niji, to see a demo, or to book your free evaluation unit please get in touch.


Modular Channels Up to seven channels – the modular chassis allows for user upgrades
Sources Solid state LED
Wavelength Range 380 – 730 nm available
Noise Level Approx. 47 dB
Bandpass Filters Individual bandpass filter holders for each channel
Light Interface Liquid light guide with microscope collimators, available separately
Connection USB -A (powered), USB-B, RS232 (serial), and TTL via a 15-way connector
Operation Control via dedicated software, MicroManager, tablet, or third party software
Programmable Commands XML upload for complete flexibility
Optical Safety Key switch, light guide, and lid-off interlock
Dimensions (L x W x H) 315 x 288 x 107 mm
Weight 4.7 kg
Warranty 12 months, optional 3 years
Light Output
UV HPUV* Royal Blue Blue Cyan Green/Yellow Red Far Red
Wavelength (nm) 395 390 445 470 505 556 635 725
Bandwidth FWHM (nm) 10 15 18 19 31 62 18 38
Typ. Optical Power (mW) 120 330 200 185 90 550 225 140
Recommended Filter Sets LED-DAPI-B-000 LED-DAPI-B-000 LED-CFP-A-000 LED-FITC-A-000 LED-YFP-A-000 LED-TRITC-A-000
LED-Cy5-A-000 LED-Cy7-A/B-000
Operating Requirements
Line Voltage 110V & 240V AC
Line Frequency 50/60 Hz
Power Consumption 350 VA
Operating Temperature 10 to 40 °C
Storage Temperature -10 to 60 °C
Humidity 40% to 70% RH

* Option requires HPUV chassis

Note: Optical output powers are given for a ø3 mm liquid light guide, use of a ø5 mm light guide will provide approximately 50% more power. Wavelengths and powers are approximate and may vary on LED batch. Each niji will be supplied with full optical power and wavelength test data.

niji – 7-colour LED Light Source for Fluorescence Microscopy

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If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.