Highly controllable LED illumination system for fluorescence microscopy

pE-300^ultra highly controllable LED illumination system – single band. Direct fit SB filter configuration. Includes light Source, control pod, set of three excitation filter holders (25mm dia.), power supply, YYY adaptor & ZZ plug

The pE-300^ultra is the most controllable member of the pE-300 Series. In addition to the many features of the pE-300^lite and the pE-300^white, the system offers precise control over wavelength intensity and shuttering. Operation is by a remote manual-control pod with instant on/off and intensity control from 0 – 100%. It allows adjustment of output in 1% steps, giving precise control. The broad spectrum covers everyday fluorophores: DAPI, cyan fluorescent protein (CFP), Aqua, fluorescein isothiocyanate (FITC), tetramethylrhodamine (TRITC), texas red, Cy5 and many more.

It is now possible to view and image your samples brighter than before.

Convaleria taken with Photometrics Prime camera with 10 ms exposure.

LED illumination means no mercury to dispose of and lower energy costs due to low power consumption and the precise control offered. The pE-300^ultra with all 3 channels enabled at full power uses just 46 Watts.

This LED light source offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light. This, when paired with today’s high performance multi-band filter sets, facilitates imaging traditionally done using a white light source and a filter wheel, with all the benefits of LED, and most excitingly at speeds not previously so affordable.

The system comprises a pE-300^ultra light source, control pod, set of three excitation filter holders, and a power supply. It can be specified with a microscope adaptor for direct fit configuration or with a 3 mm diameter liquid light guide if there is a need to keep the source of illumination remote from the microscope. An optional pE-Universal Collimator and microscope adaptor can be selected for use with a liquid light guide.

A benefit of this product is that stains can be viewed either individually or in combination, without filter cube changes. This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used. Independent control of the three LED channels means that the user can control the level of excitation of each fluorescent stain independently on a multi-stained sample, potentially removing the need for single band filter sets altogether.

An important feature is that it includes CoolLED’s Sequence Runner multiple channel excitation mode. Users can define the order of their fluorophore capture using their control pod, then the pE-300^ultra light source can accept a single TTL output from the experiment’s set-up camera to initiate the step-through of a sequence of excitation channels. This feature is independent of the individual channel TTL inputs on the light source. This offers users the facility to run through a sequence of excitation channels using a camera which has only a single TTL-out.

With an expected lifetime of over 25,000 hours and a comprehensive range of microscope adaptors, the pE-300^ultra can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.

There are two configuration options for the pE-300^ultra:

• Direct-fit for connecting to microscopes – by selecting from a range of microscope adaptors which cover all current and most older models. A simple once-only adjustment will allow optimisation to the optical path of the microscope.
• Liquid light guide with a fixed 3mm diameter. An optional pE-Universal collimator can be specified in conjunction with a microscope adaptor if required. CoolLED now includes a light source stand (see picture 3 on the product pictures).

CoolLED’s pE-300 Series was the winner of the “Go Beyond Award” in the “Products” category for “Excellence in sustainability in laboratory and other high technology facilities” The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products, and projects that are advancing sustainable, high-performance facilities.

The pE-300 Series is now ACT Label certified

With almost 40% of energy at university campuses being used by laboratories, steps need to be taken to increase the sustainability of lab technology. Introduced by My Green Lab, the ACT Label stands for accountability, consistency, and transparency. It’s a means of measuring the Environmental Impact Factor of a product by reviewing the environmental impact of the product itself, its manufacturing process, packaging, and disposal.