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BrightLine® single-band filter set: FITC and similar fluorophores, illuminated with LED-based light engines

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Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring FITC (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock’s SearchLight plotting & calculation tool.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Olympus large-size (FX) set for U-M710I
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
Fluorophore5-FAM
Cy2
DiO
FITC
GFP (EGFP)
MitoTracker Green
non-UV excitation)
sgGFP (super glow GFP)
TurboGFP
wtGFP (wild type GFP
YOYO-1


LED-FITC-A-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

LED-FITC-A-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-474/27-25 Single Band Exciter 25 x 5.0 mm 1
FF01-525/45-25 Single Band Emitter 25 x 3.5 mm 1
FF495-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-FITC-A-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

LED-FITC-A-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-474/27-25 Single Band Exciter 25 x 5.0 mm 1
FF01-525/45-25 Single Band Emitter 25 x 3.5 mm 1
FF495-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-FITC-A-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

LED-FITC-A-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-474/27-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF01-525/45-23.3-D Single Band Emitter 23.3 x 2.0 mm 1
FF495-Di03-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1

LED-FITC-A-U02

  • Size: Olympus large-size (FX) set for U-M710I
  • Filter size: Olympus ‘Large’ (FX)
  • Zero pixel shift: no

LED-FITC-A-U02 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-474/27-32 Single Band Exciter 32 x 5.0 mm 1
FF01-525/45-32 Single Band Emitter 32 x 3.5 mm 1
FF495-Di03-32×44-FX Single Band Dichroic 32 x 44 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-525_45_Spectrum
FF495-Di03_Spectrum
FF01-474_27_Spectrum

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