Microscopy Filters

Select from the full range of individual Semrock filters, according to your required parameters.

This range of high-performance, high reliability individual fluorescence bandpass filters are optimised for use in a variety of fluorescence instruments. These durable single-band and multi-band bandpass filters are hard-coated, for no burn-out performance. Most of these filters are housed in a standard 25mm round black-anodised aluminium ring with a thickness as indicated and a clear aperture of at least 21mm. Unmounted parts have part numbers that end with “-D”.

These long and short pass single-edge filters are optimised for use in a variety of fluorescence instruments. Each filter is hard-coated for no burn-out performance. The filters give the highest transmission, deepest blocking and sharpest of edge transitions to allow you to see more of your image, reveal more detail, maximise your productivity with faster image capture and preserve your data with reduced sample bleaching.

These deep notch filters rival the performance of holographic notch filters but are in a less expensive, more convenient and reliable thin-film filter format. Notch filters are ideal for applications including Raman spectroscopy, laser based fluorescence instruments, and biomedical laser systems. The filters have a high damage threshold and proven reliability. Rejected light is reflected, for easy alignment and best stray-light control. The StopLine E-grade notch filter offers high transmission over ultra-wide passbands (UV to 1600nm). The deep laser-line blocking (OD>6) gives maximum laser rejection.

Single edge and multi-edge dichroic beamsplitters are polarisation insensitive and are for use at 45°. These beamsplitters exhibit steep edges with very high and flat reflection and transmission bands. More complete reflection and transmission mean less stray light for lower background and improved signal-to-noise ratio. These filters are optimised for fluorescence microscopes and instrumentation. They are also suitable for a variety of other applications that require beam combining and separation based on wavelength. These dichroics utilise high optical quality, ultra-low-auto-fluorescence substrates. The filters are excellent for epifluorescence.

LaserMUX™ filters efficiently combine or separate multiple laser beams at a 45° angle of incidence. These dichroic laser beam combiners are optimised to multiplex (MUX) popular laser lines, and can also be used in reverse to demultiplex (DEMUX). The ultra-low autofluorescence filters are ideally suited for OEM multi-laser fluorescence imaging and measurement applications including laser microscopy and flow cytometry, as well as for myriad end-user applications in a laboratory environment.

MaxLine narrow laser-line clean-up filters have an extremely high transmission, exceeding 90% at the laser line, while rapidly rolling off to an optical density of OD >5 at wavelengths differing by just 1% from the laser wavelength. This drops to OD > 6 at wavelengths only 1.5% away from the laser wavelength. MaxDiode laser diode clean-up filters keep the desirable laser light while eliminating the noise. The square low-ripple passband gives total consistency as the laser ages, over temperature, and when replacing a laser. Transmission exceeds 90% over each diode’s possible laser wavelengths. Extremely steep edges transition to very high blocking to filter out undesired out-of-band noise.

These multiphoton ultra-high performance fluorescence filters serve a full range of applications. They accommodate the wide range of fluorescent dyes that are the essential tools of the modern researcher. The transmission bands of the emitters are so wide that they appear clear at normal incidence. The long wave pass dichroic reflection bands are so wide that they look like mirrors when viewed at 45°. These filters virtually eliminate excitation laser noise at the detector. To reduce undesired fluorescence noise outside a desired band, simply add a bandpass filter.

Select from the full range of Semrock filter sets, according to your required parameters.

We stock a wide selection of filter sets optimised for the most popular fluorophores, fluorescence microscopes, and imaging systems. High transmission, steeper edges, precise wavelength accuracy and carefully optimised blocking mean better contrast and faster measurements.

Whether you need the brightest filters available, or the most contrast, these high-performance sets meet all your needs.

The “full multiband” filter set configuration uses multiband filters for the exciter, emitter, and dichroic beamsplitter. The multiband set is ideal for direct visualisation, such as locating areas of interest on a sample. This approach to simultaneous multi-colour imaging is quick and easy to implement, and is compatible with all standard fluorescence microscopes.

The “Pinkel” filter set configuration uses single-band exciters in a filter wheel with multiband emitter and dichroic filters. It offers an economical way to achieve very high-speed, high-contrast, simultaneous multi-colour fluorescence imaging.

The “Sedat” filter set configuration uses single-band exciters and single-band emitters in synchronised filter wheels, with a multi-band dichroic beamsplitter. This approach provides the best image fidelity for high-speed simultaneous multi-colour fluorescence imaging.