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BrightLine® Sedat Multi-Band Super-Resolution Laser Filter Set – Optimized for 405, 488, 561 & 635 nm Excitation

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This BrightLine® Sedat multi-band filter set is engineered for four-band laser excitation, supporting:

  • 405 nm for DAPI
  • 488 nm for GFP or FITC
  • 561 nm for mCherry
  • 635 nm for Cy5

Designed for maximum fluorescence brightness and minimal crosstalk, this set is ideal for high-resolution imaging and super-resolution techniques such as TIRF, PALM, STORM, and Structured-Illumination.

With a 1λ P-V RWE dichroic flatness, this filter set minimizes focus shift and optical aberrations for laser beam spots up to 10 mm in diameter.

Both loose and cube-mounted configurations are available, maintaining ≤ 1λ P-V RWE @ 632.8 nm for consistent optical performance.

Compatible Cube Configurations: Available for NQF, LDMK, and LDMP cubes.

For users requiring the previous version, it remains available under SKU: LF405/488/561/635-4X4M-A.

Sustainability Note:

To reduce environmental impact, spectral plots are no longer included with shipments. If you require this data, visit our information page.

 

Optical Specifications
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Specifications
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.
Physical Specifications (for standard parts – please contact us for custom sizes)
Filter size Standard
Size Standard size (fits most microscopes)
Surface Quality (Scratch-Dig) 60-40
Exciter/Emitter Orientation Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation Reflective coating side should face toward light source and sample.
Fluorophores
Fluorophore Cy5
DAPI
FITC
GFP
mCherry
mRFP1

LF405/488/561/635-4X4M-B-000

  • Size: Standard size (fits most microscopes)

LF405/488/561/635-4X4M-B-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-390/40-25 Single Band Exciter 25 x 5.0 mm 1
FF02-482/18-25 Single Band Exciter 25 x 5.0 mm 1
FF01-563/9-25 Single Band Exciter 25 x 5.0 mm 1
FF01-640/14-25 Single Band Exciter 25 x 5.0 mm 1
FF01-445/20-25 Single Band Emitter 25 x 5.0 mm 1
FF01-525/30-25 Single Band Emitter 25 x 3.5 mm 1
FF01-605/15-25 Single Band Emitter 25 x 3.5 mm 1
FF01-676/29-25 Single Band Emitter 25 x 3.5 mm 1
Di03-R405/488/561/635-t1-25×36 Quad Band Dichroic 25.2 x 35.6 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-390_40_Spectrum
FF01-445_20_Spectrum
FF01-525_30_spectrum
FF01-563_9_Spectrum
FF01-605_15_Spectrum
FF01-640_14_spectrum
FF01-676_29_Spectrum
FF02-482_18_Spectrum
Di03-R405_488_561_635_Spectrum

* Time Limited Offer  |  Terms & Conditions Apply  |   Contact us for Further Details

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LF405/488/561/635-4X4M-B

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