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High performance laser-based IR chemical imaging microscope

Spero-QT 340

The Spero-QT 340 remains the highest-performance and most versatile infrared microscopy platform available. Alternatively, the Spero-LT 340, has been economically configured to get the most out of your research for a lower cost. With all of the same high-performance specifications in speed and resolution as the Spero-QT 340, this is a great solution for tight budgets.

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High performance laser-based IR chemical imaging microscope

The Spero-QT 340 microscope is the second-generation platform in this new class of instruments and represents yet another breakthrough in IR spectroscopy, with high-performance, label-free chemical imaging. The Spero-QT 340 system has the wide-field, high-resolution attributes of its predecessor but with the capability to produce twice the data in one-tenth of the time, while achieving unprecedented signal-to-noise ratios. The Spero-QT 340 stage can image up to 3 microscope slides, and its larger sample compartment makes the Spero-QT 340 more compatible with microfluidic devices and accessories.

Features & Benefits

  • Real-time, high-throughput spectral imaging enabled by ultra-high brightness QCL technology
  • No stains or molecular tags required
  • Label-free IR spectroscopy displays pure chemistry of sample
  • No cryogenic cooling needed
  • Quick setup means more time for analysis

Applications

  • Biomedical imaging of tissues, cells, and fluids
  • Cancer research
  • Pharmaceutical testing of tablets, powders, and liquids
  • Drug discovery: API and excipient optimisation and down-selection
  • Protein secondary structure and aggregation testing
  • Real-time reaction monitoring
  • Materials testing and analysis
  • Forensics
  • Chemical detection and identification
IR Imaging Mode
Parameter High-resolution IR (0.7 NA) [1] Wide-field IR (0.3 NA)
Wavelength Range: Standard configuration: 1800 cm-1 to 950 cm-1
Other wavelength range options available between 2300 cm-1 and 800 cm-1 Please enquire.
Image Cube Acquisition Time: < 40 s (450 absorbance images collected at 2 cm-1 spacing)
Camera Array Size: 480 x 480 480 x 480
Image Pixel Size: 1.3 μm (0.7 NA) 4.3 μm (0.3 NA)
Diffraction-Limited Spatial Resolution: < 5 μm @ λ = 5.5 μm < 12 μm @ λ = 5.5 μm
Numerical aperture (NA): 0.7 0.3
Spectral Resolution: Variable, down to 2 cm-1
Minimum Detectable Signal: < 1 mAU per scan [4]
Working distance (WD): >8 mm >25 mm
Field of view (FOV): 650 µm x 650 µm (0.7 NA) 2 mm x 2 mm (0.3 NA)

 

Stage
Stage Travel X > 75 mm [3]
Stage Travel Y > 50 mm [3]
Stage Travel Z > 10 mm
Stage Repeatability < 1 µm
[1] Reflection mode not included in standard configuration of Spero-LT.
[2] High-Resolution IR Objective and visible objective not included in standard configuration of Spero-LT.
[3] Customisable up to 100 mm
[4] IR imaging mode
Note: Dry gas purge recommended. Please contact us for installation recommendations.

Configurations

Imaging Modes Spero-LT 340 Spero-QT 340
IR Reflection Yes
IR Transmission Yes Yes
Visible Yes
Mosaic Stitching Yes Yes
Hypercube Collection Yes Yes
High Resolution IR Objective (0.7 NA) Yes
Wide-field IR Objective (0.3 NA) Yes Yes
Spero-QT / Spero-LT Datasheet
A-protocol-for-rapid-label-free-histochemical-imaging-of-fibrotic-liver-Research-Paper
Fourier transform vs quantum cascade laser infrared microscopes for histo-pathology. From lab to hospital. (research paper)
High definition infrared chemical imaging of colorectal tissue using a Spero QCL microscope. (research paper)
Real-time mid-IR chemical imaging of dynamic processes. Proton Dueteron exchange within a micro fluidic system using the Spero QCL. (research paper)
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Spero-QT 340

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