Laser 2000

Re-scan Confocal Microscope Upgrade

Please select either RCM-VIS (visible) or RCM-NIR (near infrared) and choose a Promo:

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RCM1 – Stock Clearance Promotion

Did you ever want a Confocal super-resolution on your existing widefield microscope, but did not have the budget? Then now is your chance with up to 50% discount on the RCM 1! Contact us for your personal offer!

RCM1 is a highly sensitive super resolution confocal add on system. It’s capable of reaching a 120 nm resolution after deconvolution (170 nm raw). Studying living cells in high details with minimal phototoxicity and photobleaching.

RCM1 enables you to investigate live-cell dynamics in high resolution while keeping the laser power extremely low. Its high sensitivity makes RCM exceptionally suitable for challenging biological applications where the signal is limited.

RCM1-NIR version facilitates deep tissue biomedical imaging, achieving much higher sensitivity in the near-infrared wavelengths than other systems. It works with 640 nm and 785 nm wavelengths which, due to the longer wavelength, are less phototoxic and penetrate much better into biological samples.

Promotion Content Price
Promo A RCM1 unit, Nidaq card and trigger cables Please select either the Visible or NIR model and your preferred promo option above, and request a quote.
Promo B Promo A + including Lasercombiner 405, 488, 561, 638 Tucson FL20BW camera
Promo C Promo A + Lasercombiner 405, 488, 561, 638 Prime BSI Express camera

 

Working principle of the re-scan confocal microscope

Re-scan Confocal Microscopy (RCM) is a super-resolution technique based on a standard laser-scanning confocal system, extended with an optical re-scanning unit. The re-scanner writes the image directly onto a camera chip. By doubling the sweep of the re-scanning mirrors, the image is magnified on the camera chip without increasing the size of the re-scanning spot. This results in an increase in resolution up to 170 nm. The camera-based detection and open pinhole design offer a high signal-to-noise ratio, while maintaining confocal sectioning capability. The re-scanning principle is optics-mechanics only, achieving an improved resolution image without any post-processing.

To find out how the RCM can benefit your project, watch this detailed video (8:22 mins) on how it works:

We recommend using the ORCA-Flash4.0 V3 Digital CMOS camera from Hamamatsu with this product. For more camera recommendations please go to the downloads section of this product page.

Better signal-to-noise ratio

The unique open pinhole design and camera-based detection allow twice the QE (up to 95%) and a 4x improvement of the signal-to-noise ratio.

Improved lateral resolution

RCM offers up to 170 nm lateral resolution, 1.4x improvement over a regular confocal microscope. Further improvement possible with deconvolution, up to 120nm.

User-friendly operation

High-resolution images are achieved with one-click; there are no adjustable settings, and no post-processing is required.

Open system architecture

Easy integration with your preferred system components; microscope and camera connect via C-mount, laser via single mode fibre. Both commercial and open-source software drivers are available.

Affordability

Low-cost due to its unique camera-based design and flexible system architecture.

Long-term mitochondria imaging with no signs of phototoxicity.

Detector

Camera

Resolution

Visible 170 nm raw image (120nm after deconvolution), NIR 240 nm raw image

Quantum Efficency

80-95 % (depending on camera)

Field of View

130 x 130 μm (60x, super-resolution)

Optimised for

100x, 60x (high NA)

Scan Speed

Typical 1 fps at 512 x 512 images

Model

Near Infrared, Visible

Software

Micromanager, Nis Elements, Volocity

PSF for deconvolution

Microvolution, SVI Huygens

Bypass mode

Yes

Confocal.nl RCM Brochure
Advantages of RCM vs PMT based Confocal Microscopes
White paper - Long term live cell imaging
Top Applications
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RCM1

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If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.