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640 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

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BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems. This dichroic beamsplitter is optimized for reflecting imaging beams up to 10 mm in diameter while minimizing RWE.

Common Fluorophore Pair to Split: Cy3/Cy5

Interested in an improved Flatness / RWE version? Consider Semrock’s image-splitting dichoric beamsplitter for super-resolution microscopy:
λ/5 P-V RWE on 3mm FF640-FDi02-t3, optimized for reflecting imaging beams up to 37 mm in custom size versions.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Transmission BandTavg > 93% 652 – 950 nm
Reflection BandRavg > 95% 350 – 629.5 nm
Edge Wavelength640 nm
Common Fluorophore Pair to SplitCy3/Cy5

General Specifications
Angle of Incidence45 ± 1.5 °
Cone Half-angle2 °
Filter Effective Index1.74 Understanding 'Effective Index of Refraction' neff
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.
Flatness / RWE ClassificationImage-splitting
Reflected Wavefront Error< 2λ P-V RWE @ 632.8

Physical Specifications (for standard parts - please contact us for custom sizes)
Mounted / UnmountedUnmounted
Size25.2 x 35.6 x 1.1 mm
Transverse Dimensions, LxW25.2 mm x 35.6 mm
Transverse Tolerance, Mounted± 0.1 mm
Filter Thickness, Unmounted1.05 mm
Filter Thickness Tolerance, Unmounted± 0.05 mm
Clear Aperture≥ 80% (elliptical)
Surface Quality (Scratch-Dig)60-40
Substrate Thickness1.05 mm
Substrate TypeLow-autofluorescence optical quality glass
OrientationReflective surface marked with part number – Orient in direction of incoming light


  • Size: 25.2 x 35.6 x 1.1 mm


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Optical Density

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Semrock Spectral Plot Info

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