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405/488/561/635 nm lasers BrightLine® quad-edge super-resolution / TIRF dichroic beamsplitter

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The perfect beamsplitters for the most popular lasers used in fluorescence imaging, including all-solid-state lasers. All beamsplitters in this category have exceptional reflectance at the key laser wavelengths, and wider reflection bands — into UV for photoactivation and super-resolution techniques. Additionally, they feature extended transmission regions — into IR to 1200 or 1600 nm, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light.Semrock’s super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and Super-resolution techniques such as TIRF, PALM, STORM, Structured Illumination, and STED. 1λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWEλ/5 P-V RWE on 3 mm, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE

Optical Specifications
Transmission BandBand 1: Tavg > 93% 426 – 462 nm
Band 2: Tavg > 93% 502.5 – 544.5 nm
Band 3: Tavg > 93% 582 – 617.5 nm
Band 4: Tavg > 93% 663 – 1200 nm
Reflection BandBand 1: Rabs > 94% 370 – 410 nm
Band 2: Rabs > 94% 473 – 491 nm
Band 3: Rabs > 94% 559 – 568.2 nm
Band 4: Rabs > 94% 632.8 – 647.1 nm
Band 5: Ravg > 90% 350 – 370 nm
Reflection Band, p-polRabs > 90% 370 – 410 nm
Rabs > 90% 473 – 491 nm
Rabs > 90% 559 – 568.2 nm
Rabs > 90% 632.8 – 647.1 nm
Reflection Band, s-polRabs > 98% 370 – 410 nm
Rabs > 98% 473 – 491 nm
Rabs > 98% 559 – 568.2 nm
Rabs > 98% 632.8 – 647.1 nm
Edge WavelengthBand 1: 418.5 nm
Band 2: 498.3 nm
Band 3: 576.5 nm
Band 4: 658.1 nm


General Specifications
Angle of Incidence45 ° with a shift of 0.35%/° (40 – 50 °)
Cone Half-angle0.5 °
SteepnessSteep
Filter Effective Index2.05 Understanding 'Effective Index of Refraction' neff
Optical Damage Rating1 J/cm sq. @ 532 nm (10 ns pulse width)
Flatness / RWE ClassificationSuper-resolution / TIRF


Physical Specifications (for standard parts - please contact us for custom sizes)
Transverse Dimensions, LxW25.2 mm x 35.6 mm
Clear Aperture≥ 80% (elliptical)
Surface Quality (Scratch-Dig)60-40
Substrate TypeFused Silica
OrientationReflective surface has laser dot–Orient in incoming light


Di03-R405/488/561/635-t1-25x36

  • Filter Thickness Tolerance, Unmounted: ± 0.05 mm
  • Filter Thickness, Unmounted: 1.05 mm
  • Search-Application: General Laser & Optical Systems
  • Search-Laser-Wavelength: 372 nm,375 nm,405 nm,418 nm,473 nm,488 nm,491 nm,497 nm,541 nm,635 nm
  • Search-Light Source: Laser Diode
  • Reflected Wavefront Error: < 1 P-V RWE @ 632.8
  • Laser Wavelength: 405 nm
    488 nm
    491 nm
    635 nm

Di03-R405/488/561/635-t3-25x36

  • Filter Thickness Tolerance, Unmounted: ± 0.1 mm
  • Filter Thickness, Unmounted: 3.0 mm
  • Search-Application: General Laser & Optical Systems,Super-resolution,TIRF
  • Search-Laser-Wavelength: 372 nm,375 nm,405 nm,418 nm,473 nm,488 nm,491 nm,497 nm,541 nm,635 nm,659 nm
  • Search-Light Source: Laser Diode,Precise Laser Line
  • Reflected Wavefront Error: < 0.2 P-V RWE @ 632.8
  • Laser Wavelength: 372 nm
    375 nm
    405 nm
    473 nm
    488 nm
    491 nm
    635 nm

Transmission

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Optical Density

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Semrock Spectral Plot Info
Di03-R405_488_561_635_Spectrum

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