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405/488/561/635 nm lasers BrightLine® quad-edge super-resolution / TIRF dichroic beamsplitter

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The perfect beamsplitters for the most popular lasers used in fluorescence imaging, including all-solid-state lasers. All beamsplitters in this category have exceptional reflectance at the key laser wavelengths, and wider reflection bands — into UV for photoactivation and super-resolution techniques. Additionally they feature extended transmission regions — into IR to 1200 or 1600 nm, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light.

Semrock’s super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and Super-resolution techniques such as TIRF, PALM, STORM, Structured Illumination, and STED.

1λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3 mm, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Transmission BandBand 1: Tavg > 93% 426.0 – 462.0 nm
Band 2: Tavg > 93% 502.5 – 544.5 nm
Band 3: Tavg > 93% 582 – 617.5 nm
Band 4: Tavg > 93% 663 – 1200 nm
Reflection BandBand 1: Rabs > 94% 370 – 410 nm
Band 2: Rabs > 94% 473 – 491 nm
Band 3: Rabs > 94% 559 – 568.2 nm
Band 4: Rabs > 94% 632.8 – 647.1 nm
Band 5: Ravg > 90% 350 – 370 nm
Reflection Band, p-polBand 1: Rabs > 90% 370 – 410 nm
Band 2: Rabs > 90% 473 – 491 nm
Band 3: Rabs > 90% 559 – 568.2 nm
Band 4: Rabs > 90% 632.8 – 647.1 nm
Reflection Band, s-polBand 1: Rabs > 98% 370 – 410 nm
Band 2: Rabs > 98% 473 – 491 nm
Band 3: Rabs > 98% 559 – 568.2 nm
Band 4: Rabs > 98% 632.8 – 647.1 nm
Laser WavelengthBand 1: 375 nm ± 3
Band 1: 405 nm ± 5
Band 2: 473 nm +2/-0
Band 2: 488 nm +3/-2
Band 3: 559 nm +5/-0
Band 3: 561.4 nm
Band 3: 568.2 nm
Band 4: 632.8 nm
Band 4: 635 nm +7/-0
Band 4: 647.1 nm
Edge WavelengthBand 1: 418.7 nm
Band 2: 498.3 nm
Band 3: 575.4 nm
Band 4: 655.3 nm


General Specifications
Angle of Incidence45 ° with a shift of 0.35%/° (40 – 50 °)
Cone Half-angle0.5 °
Filter Effective Index2.05 Understanding 'Effective Index of Refraction' neff
Optical Damage Rating1 J/cm² @ 532 nm (10 ns pulse width)
Flatness (3 mm)1/5 wave PV RWE @ 632.8 on 3 mm
Flatness / RWE ClassificationSuper-resolution / TIRF
Reflected Wavefront Error< 0.2λ P-V RWE @ 632.8
< 1λ P-V RWE @ 632.8


Physical Specifications (for standard parts - please contact us for custom sizes)
Mounted / UnmountedUnmounted
ShapeRectangular
Size25.2 x 35.6 x 1.1 mm
25.2 x 35.6 x 3.0 mm
Transverse Dimensions, LxW25.2 mm x 35.6 mm
Transverse Tolerance, Mounted± 0.1 mm
Substrate Thickness Tolerance, Unmounted± 0.05 mm
Clear Aperture≥ 80% (elliptical)
Surface Quality (Scratch-Dig)60-40
Substrate TypeLow-autofluorescence optical quality glass


Di03-R405/488/561/635-t1-25x36

  • Filter Thickness Tolerance, Unmounted: ± 0.05 mm
  • Filter Thickness, Unmounted: 1.05 mm
  • Size: 25.2 x 35.6 x 1.1 mm
  • Substrate Thickness: 1.05 mm

Di03-R405/488/561/635-t3-25x36

  • Filter Thickness Tolerance, Unmounted: ± 0.1 mm
  • Filter Thickness, Unmounted: 3.0 mm
  • Size: 25.2 x 35.6 x 3.0 mm
  • Substrate Thickness: 3.0 mm

Transmission

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Optical Density

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Semrock Spectral Plot Info
Di03-R405_488_561_635_Spectrum
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Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.