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405/488/561/635/800-1050 nm lasers BrightLine® multi-edge multiphoton super-resolution / TIRF dichroic beamsplitter

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Innovative dichroic beamsplitter designed to simplify the combination of 1P confocal imaging and 2P excitation for multiphoton applications. This dichroic beamsplitter includes reflection bands for the most popular lasers used in fluorescence imaging, including all-solid-state lasers. The 405 nm reflection is optimal for imaging or uncaging applications. The 2P excitation region is ideal for femtosecond pulsed lasers such as Ti:Sapphire, OPO coupled, and Ytterbium-doped lasers, enabling deep tissue imaging with improved contrast. This multiphoton dichroic has low reflected GDD that helps minimize temporal pulse broadening of reflected laser beams, and have steep edges with very high and flat reflection and transmission bands.

Semrock’s super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and super-resolution techniques such as TIRF, PALM, STORM, structured illumination, and STED.

2.5λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 6 mm in diameter while minimizing RWE
λ/3 P-V RWE on 3 mm, optimized for reflecting laser beams up to 16.7 mm in diameter while minimizing RWE

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Transmission BandBand 1: Tavg > 92% 425 – 470 nm
Band 2: Tavg > 92% 508 – 540 nm
Band 3: Tavg > 92% 583 – 615 nm
Band 4: Tavg > 92% 671 – 725 nm
Reflection BandBand 1: Rabs > 94% 400 – 410 nm
Band 2: Rabs > 94% 483 – 493 nm
Band 3: Rabs > 94% 559 – 563 nm
Band 4: Rabs > 94% 635 – 647 nm
Band 5: Ravg > 94% 800 – 1050 nm
Reflection Band, p-polBand 1: Rabs > 90% 400 – 410
Band 2: Rabs > 90% 483 – 493
Band 3: Rabs > 90% 559 – 563
Band 4: Rabs > 90% 635 – 647
Band 5: Ravg > 90% 800 – 1050
Band 5: Ravg > 98% 800 – 1050
Reflection Band, s-polBand 1: Rabs > 98% 400 – 410
Band 2: Rabs > 98% 483 – 493
Band 3: Rabs > 98% 559 – 563
Band 4: Rabs > 98% 635 – 647
Laser WavelengthBand 1: 405 nm ± 5
Band 2: 488 nm ± 5
Band 3: 559 nm +4/-0
Band 3: 561.4 nm
Band 4: 635 nm +7/-0
Band 4: 642 nm ± 5
Band 5: 800 - 1050 nm
Edge WavelengthBand 1: 420 nm
Band 2: 501 nm
Band 3: 577 nm
Band 4: 662 nm
Band 5: 735 nm


General Specifications
Angle of Incidence45 ° with a shift of ~0.35%/° (40 – 50 °)
Cone Half-angle0.5 °
Filter Effective Index1.87 Understanding 'Effective Index of Refraction' neff
Group Delay Dispersion± 500 fs² over 800 - 1050 nm reflection band for S-Pol and P-Pol
Optical Damage Rating1 J/cm² @ 532 nm (10 ns pulse width)
Flatness / RWE ClassificationSuper-resolution / TIRF
Reflected Wavefront Error< 0.33λ P-V RWE @ 632.8
< 2.5λ P-V RWE @ 632.8


Physical Specifications (for standard parts - please contact us for custom sizes)
Mounted / UnmountedUnmounted
ShapeRectangular
Size25.2 x 35.6 x 1.1 mm
25.2 x 35.6 x 3.0 mm
Transverse Dimensions, LxW25.2 mm x 35.6 mm
Transverse Tolerance, Mounted± 0.1 mm
Substrate Thickness Tolerance, Unmounted± 0.05 mm
Clear Aperture≥ 80% (elliptical)
Surface Quality (Scratch-Dig)60-40
Substrate TypeLow-autofluorescence optical quality glass


Di01-R405/488/561/635/800-t1-25x36

  • Filter Thickness Tolerance, Unmounted: ± 0.05 mm
  • Filter Thickness, Unmounted: 1.05 mm
  • Size: 25.2 x 35.6 x 1.1 mm
  • Substrate Thickness: 1.05 mm

Di01-R405/488/561/635/800-t3-25x36

  • Filter Thickness Tolerance, Unmounted: ± 0.1 mm
  • Filter Thickness, Unmounted: 3.0 mm
  • Size: 25.2 x 35.6 x 3.0 mm
  • Substrate Thickness: 3.0 mm

Transmission

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Optical Density

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Semrock Spectral Plot Info
Di01-R405_488_561_635_800-1050_Spectrum
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